Principles of Microbiology ( Official )
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Principles of Microbiology ( Official )
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Principles of Microbiology ( Official )

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SECTION A – Course Information

1. Course ID:

MICR     1

Student Learning Outcomes

2. Course Title:

Principles of Microbiology

3. Division:

Natural Sciences Division

4. Department:

Biological Sciences Department

5. Subject:


6. Short Course Title:


7. Proposed Effective Term:

Summer 2016

The required Cover Sheet Supplemental Form can be created after completion of Section A

SECTION B – Official Course Information

1. Recommended Class Size:

a. Maximum Class Size:


Note:   If the course is new or class size of an existing course is to be changed, a Class Size Supplemental Form is required.

b. Class Size Approval Date:


Note:   Date to be entered by the Instruction Office.


2. Method of Instruction:


 Work Experience, Occupational


 Open Entry/Exit

 Lecture and Laboratory


 Independent Studies

 Distance Learning



3. Contact Hours for a Term:

Note: If not a variable unit/hour course, enter the hours in the "Low" column only. Leave the hours in the "High" column blank.






         54.00 To


To be arranged:






       108.00 To


Lab/Lecture Parity?    Yes  No

Does this course have lab parity?

If not, are you going to  apply for lab parity?                          Yes  No

To be arranged:







To be arranged:







Total Hours:    


            162 To


To be arranged:





4. Credit Units:

  5.00 To


Note: Units of credit are based on:

1 Unit of credit per eighteen (18) hours of lecture contact hours for a term

1 Unit of credit per fifty-four (54) hours of lab, activity or clinical contact hours for a term


5. Taxonomy of Programs (TOPS) Information:

a. TOPS Code and Course Program Title:

040100 - Biology, General

     b.  Course Control Number:    

(To be entered by the Instruction Office Only.)


6. SAM Priority Code: [Select One]



Courses offered to apprentices only.


Advanced Occupational

Courses taken in the advanced stages of an occupational program. Each “B” level course must have a “C” level prerequisite in the same program area.


Clearly Occupational

Courses taken in the middle stages of an occupational program. Should provide the student with entry-level job skills.


Possibly Occupational

Courses taken in the beginning stages of an occupational program.





7. Please place this course into the appropriate discipline by selecting from the drop down list. The discipline placement indicates what preparation is needed to teach the course. Discipline faculty may place their courses into more than one discipline as appropriate:

Biological Sciences

8. General Course Information

a. Course Credit Status:

D Credit Degree Applicable

b. State Transfer Code:

A Transferable, UC/CSU/Private

c. State Classification Code:

A Liberal Arts/Sciences Degrees

d. Basic Skills Status/Level:

N Not a Basic Skills Course

e. Sports/Physical Education Course:


( Only check here if the course is a physical education course.)

f. Grading Method:

Letter Grade Only

g. Number of repeats allowed:

Non-repeatable Credit - equates to 0 repeats

Note: If course is repeatable, complete the Repeatability Supplemental Form.

h. Please provide cross listed course if applicable:



9. Course Preparation

Note: If this course has any requisite, please complete the Content Review Supplemental Form and provide rationale for the requisite. If a requisite is being removed, please complete the Content Review Supplemental Form and provide rationale for removing the requisite. If a new requisite is being added, complete the Content Review Supplemental Form and provide rationale for the requisite.

a.   Prerequisite

CHEM 10 or CHEM 40

b.   Co requisite

c.   Advisories

d.   None


10. Course Special Designators:

Do not edit this text box. Use the 'Add'/'Remove' command buttons above.

11. Course Program Status:

These buttons are only active when the course reaches Stage 5 in WebCMS.


 Program Applicable


 12. Funding Agency Category:

       Not Applicable

       Primarily developed using economic development funds

       Partially developed using economic development funds



SECTION C – For new transfer requests only, please complete the Transfer Status (CSU) Supplemental Form before submitting course for approval.

CSU Transferable              CSU Approval Date:                     (mmddyyyy)                 

Applying for CSU Transfer Status

UC Transferable                 UC Approval Date:                      (mmddyyyy)                 

Applying for UC Transfer Status

Note:  CSU Transfer Status must be obtained prior to submitting a request for UC Transfer Status to the UC Chancellor.


SECTION D - General Education Request

Mt. San Antonio College and CSU General Education course approvals are submitted to the Educational Design Committee and GE Subcommittee for approval.

1.  The Articulation Officer submits the course directly to the CSU Chancellor for approval.

2.  Upon receiving CSU approval, the course will be placed in the CSU approved area for the Mt. SAC Associate Degree GE.



Requesting approval for inclusion on Mt. SAC and CSU General Education List?

Note:   If requesting approval for inclusion on the General Education List, the General Education Course Evaluation Supplemental Form must be completed before submitting course for approval.  If request is approved, the remainder of Section D will be completed by the Instruction Office.

1. Mt SAC General Education Applicability:

    GE Approval Date :                   (mmddyyyy)

AAB2 - Life Sciences
ASB2 - Life Sciences

2. CSU General Education Applicability (Requires CSU approval):

    CSU Approval Date:                   (mmddyyyy)

CSB2 - Life Science
CSB3 - Laboratory Activity

3. IGETC Applicability (Requires CSU/UC approval):

    IGETC Approval Date:                   (mmddyyyy)

IG5B - Biological Science - Lecture Only
IG5C - Physical Science Combo



SECTION E - Course Content

1. Course Descriptions

a. Catalog Description:

(Write a clear, concise course description, summarizing the course content.  Include major goals of the course, scope, requirements for successfully completing the course, and any unusual aspects of the course.)

Fundamental concepts of microbiology with emphasis on bacteria. Survey of microbial classification, morphology, physiology, and genetics; beneficial and pathological aspects; growth and control of microbes; virology, immunology, and host-microbe interactions. Important infectious diseases of humans are surveyed. Laboratory exercises examine microbial morphology, physiology, and genetics as well as environmental influences of microorganisms. Laboratory techniques include culturing, examining, and identifying microorganisms. Field trips are required.

b. Class Schedule Description:



Is a course description to be printed in the Class Schedule?

(If yes, write one or two sentences condensing the catalog description for the prospective student.  Does not require as much detail as the catalog description. Limited to 130 characters, including spaces.)

Microbial classification, physiology, genetics, immunology, and host-microbe interactions. Field trips required.

2. Course Outline Information

In courses that include lecture and laboratory, the topical outlines should be separate and distinct, each specific and appropriate to the activities to be conducted.

a. Lecture Topical Outline:

(The lecture topical outline should provide a detailed record of the content of the course.)

- Scope, history, and development of microbiology
- Basic concepts of microbiology
- Properties of microorganisms; microscopy; microbes in the scheme of life: the kingdom classification system and bacterial taxonomy
- Characteristics of prokaryotic cells
- External and internal structures and functions of a typical bacterial cell
- Comparisons and contrasts to eukaryotic cells
- Bacteria: gram positive and negative bacteria, atypical and unusual bacteria
- Growth and culturing bacteria
- Factors affecting bacterial growth
- Viruses: structure, replication, diseases, control, classification, viroids, prions
- Eukaryotic microorganisms and parasites: fungi, algae, protozoa, and parasitic worms
- Microbial metabolism: enzymatic function, anaerobic and aerobic pathways of energy production
- Microbial metabolism: utilization of energy, anabolism of carbohydrates, anabolism of proteins
- Microbial genetics I: gene action, gene regulation, and mutation in prokaryotic cells
- Microbial genetics II: transfer of genetic material (bacterial recombination) and genetic engineering: recombinant deoxyribonucleic acid (DNA) and biotechnology
- Infection and disease: host-microbe relationships and disease processes
- Epidemiology and nosocomial infections
- Host systems and non-specific host defenses
- Immunology: basic principles, immunity, and serology
- Immune disorders
- Principles of sterilization and disinfection
- Control of microbes by chemical and physical agents
- Final exam

b. Lab Topical Outline:

(The lab topical outline should reflect the activities in lab.)

- Exploring the microbiology laboratory
- Using and caring for microscopes
- Using aseptic techniques, methods of live culture handling, and culturing the environment
- Preparing bacterial smears and staining: simple, capsular, spore, and gram-staining
- Acid-fast staining and motility determination: hanging drop and stab techniques
- Conducting a morphological study of unknown #1
- Conducting bacterial population counts: quantitative plating and turbidity measurements
- Conducting pure culture techniques
- Investigating temperature: lethal effects
- Measuring pH and microbial growth
- Determining osmotic pressure effects on bacterial growth
- Observing lethal effects of ultraviolet light
- Observing oligodynamic action
- Cultivating anaerobes
- Identifying protozoa and diseases they cause
- Identifying fungi: yeasts, molds, and fungus-like bacteria
- Identifying parasitic worms and diseases they cause
- Identifying biotechnology: principles and practice of agarose gel electrophoresis
- DNA fingerprinting
- Transforming E coli cells with plasmid DNA
- Identifying Unknown #2: preparation and care of stock cultures, cultural characteristics, physiological characteristics, and biooxidation
- Conducting a morphological study
- Identifying Unknown #2: physiological characteristics: hydrolysis, miscellaneous tests
- Applying data applications to systematics: use of Bergey's Manual and Identibacter Interactus CD-ROM for unknown #2 identification
- Using selective and differential media, primary isolation media, urine sediment examination, and plate count cultures
- Using multitest media: enterotube II system or Analytical Profile Index (API) 20E system for enterobacteriaceae identification
- Conducting bacterial counts of foods
- Exploring the microbiology of yogurt production
- Visiting a microbiology labor research facility
- Simulating sexually transmitted disease
- Examining immunobiotechnology: Acquired Immune Deficiency Syndrome (AIDS) kit 1, simulation of Human Immunodeficiency Virus (HIV)-1 detection, and Enzyme-Linked Immunosorbant Assay (ELISA)
- Evaluating antiseptics: alcohol evaluation as a degerming agent
- Antimicrobic sensitivity testing
- Completing a lab practicum pathogen test
- Final exam

3. Course Measurable Objectives:

(Measurable course objectives should identify expected outcomes: specific, observable student actions – what the student should be able to do, know or feel as a result of taking this course.  A majority of objectives should reflect critical thinking, i.e. application, analysis, synthesis, and evaluation.  Course objectives should relate directly to methods of evaluation)

Provide a minimum of five (5) course measurable objectives:

1. Describe the structures and functions of external and internal components of both prokaryotic and eukaryotic cells.
2. Draw standard growth curves for bacterial cultures and explain factors affecting bacterial growth.
3. Describe characteristics of selected pathogens and the diseases caused by each.
4. Describe the role of genes, chromosomes, mutations, and human manipulation in heredity of prokaryotic cells.
5. Contrast the metabolic processes of fermentation and aerobic metabolism noting cycles involved, energy production, and end products.
6. Outline the general characteristics of viruses, prions, and viroids.
7. Evaluate the best method to control microbes in various settings such as chemical, physical, or chemotherapeutic agents.
8. Describe the course of infectious diseases including the interactions with host defenses.
9. Identify the fundamental concepts of immunity, immunization, immune deficiencies, and immunological testing.
10. Demonstrate aseptic technique and safe handling of microbial cultures.
11. Prepare smears, perform staining procedures, and record microscopic observations.
12. Identify an unknown bacterial organism based on results of lab procedures performed and through a miniaturized multitest system and compare findings of these two methods.
13. Evaluate physical, chemical, and chemotherapeutic agents.
14. Evaluate the level of contamination in water, milk, and specific food products.
15. Perform quantitative plating and turbidity measures to determine the number of bacteria present in a culture sample.
16. Describe and accurately draw various microbes based on microscopic observations.
17. Perform a molecular separation technique (gel electrophoresis) and identify a DNA source using DNA fingerprinting.
18. Observe bacterial transformation by plasmid DNA and describe an acquired phenotypic trait of the transformed cells.
19. Use an immunobiotechnological procedure (ELISA) to detect a positive HIV reaction in a simulation.

4. Course Methods of Evaluation:

(Methods of evaluation should relate directly to measurable course objectives.  They indicate the kind of assignments or performance activities designed for a course to assess student learning.  Each course must list either substantial writing assignments (category 1) OR computational / non-computational problem solving demonstrations (category 2) if writing assignments are inappropriate (with an explanation of why substantial writing is not appropriate for the course).  Activities typically assigned in categories 3 and 4 must also be listed in this section.)

Category 1. Substantial written assignments for this course include:

Ten two- to three-page laboratory reports
Five one- to three-page summaries of current issues in medical microbiology from the popular literature or news reports, discussions of ethical topics in microbiology, evaluations of new diagnostic techniques
Five to ten page term or other paper may be assigned to explore topics that have evolved significantly in recent history, such as antibiotic resistance and emerging infectious diseases
64 one-page pathogen charts including research of each pathogen and information in specific format to be used for the pathogen test

If the course is degree applicable, substantial written assignments in this course are inappropriate because:

Category 2. Computational or non-computational problem solving demonstrations:

Completion of biochemical laboratories requiring mathematical calculations including pH, concentration, and dilutions
Analysis of data and an explanation of any unexpected or inaccurate results
Completion of problem(s) include metric conversion problems, calculation of pH, and calculation of dilutions and microbial concentration in initial cultures
Evaluation of medical case studies that determine the causative agent and the disease
Identification of a series of slides or organisms growing in culture based on their appearance

Category 3. Skills Demonstrations:

Lab technique demonstration of the ability to use the microscope correctly and to prepare slides using a variety of staining techniques

Category 4. Objective Examinations:

Multiple choice, completion, short answer, and true or false questions covering pathogens, disease symptoms, technical terminology, and case studies
Matching lab tests to specimens
Safety exams on laboratory techniques

5. Sample Assignments:

(Assignments should be directly related to the objectives of the course.  They should be specific enough to provide real guidance to faculty and clear expectations for students.  Descriptions of the type or examples of assignments are required.  For example, rather than “term paper” state “term paper comparing and contrasting the social aspects of hunting tactics of two mammal species.”  This section must establish that the work is demanding enough in rigor and independence to fulfill the credit level specified.  The nature of the assignments must clearly demand critical thinking.  Assignments should be adequate to assure that students who successfully complete them can meet the objectives of the course.  Appropriate out-of-class work is required for credit courses.)

Provide a minimum of three (3) sample assignments:

1. Evaluate and summarize an article from the primary literature and prepare a 15-minute oral presentation for Journal Club using PowerPoint.
2. Prepare a five-page written report in which information from course and reading assignments are used to suggest disease etiology for a medical case study.
3. Use biochemical assays, staining techniques, and visual observation to determine the identity of a microbiological unknown.
4. Diagram three main mechanisms of genetic material transfer and discuss the significance of each.


6. Representative Text:

(List EXAMPLES of textbooks and other data sources and materials, which may be used in this course.  Provide author, title, publisher, date of publication, and edition.)

If the course is requested to be or is CSU transferable, provide at least one (1) representative text that has been published within the last five (5) years.  A representative text is optional for a non-transferable course.

a. Book 1:

This is the most recent edition of this text:




If the text is more than 5 years old, please provide rationale for not selecting a more recent text:


  Murray, Patrick R & Rosenthal, Ken S.


  Medical Microbiology



Date of Publication:




b. Book 2:

This is the most recent edition of this text:




If the text is more than 5 years old, please provide rationale for not selecting a more recent text:


  Heymann, David


  Control of Communicable Diseases Manual


  APHA Press

Date of Publication:

  October 30, 2014



c. Book 3:

This is the most recent edition of this text:




If the text is more than 5 years old, please provide rationale for not selecting a more recent text:







Date of Publication: